Acrosome Integrity Spermatozoa Test with Addition of Gentamicin and Sweet Orange Essential Oil in Simmental Bull Liquid Semen
نویسندگان
چکیده
منابع مشابه
The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa
The aim of this study was to determine the effects of the antioxidants superoxide dismutase (SOD),glutathione peroxidase (GPx) and butylated hydroxytoluene (BHT) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. Ejaculates were collected from five Holsteinbulls and pooled at 37°C. The semen samples were diluted with a CEY extender containing addit...
متن کاملInfluence of Boar and Semen Parameters on Motility and Acrosome Integrity in Liquid Boar Semen Stored for Five Days
Ninety ejaculates from a total of 76 AI boars were extended in Beltsville Thawing Solution (BTS). Boar identity, breed, weight of the ejaculate and sperm concentration were registered. Motility and acrosome integrity were assessed after storage at 16-18 degrees C for 6, 30, 54, 78, and 102 h. Storage time had a significant influence on both motility (p < 0.01) and acrosome integrity (p < 0.001)...
متن کاملEffects of addition of vitamin B12 to the extender on post-thaw motility, acrosome morphology, and plasma membrane integrity in bull semen
To investigate the effects of supplementation of vitamin B12 on bovine sperm post-thaw quality, vitamin B12 was added at the concentrations of 1.25, 2.50, 3.75, and 5.00 mg/mL to bovine semen cryoprotective medium. The results indicated that the motility and VSL, VCL, STR, and VAP values of sperm supplemented with 2.50 mg/mL vitamin B12 were significantly higher than those of other concentratio...
متن کاملIdentification of Heparin Binding Proteins in Frozen-thawed Capacitated/Acrosome-reacted Spermatozoa and Their Relationship with Fertility of Buffalo Bull Semen
The present was conducted to separate heparin binding proteins (HBP) of capacitated sperm extracts and determine their relationship with acrosome reaction and semen fertility of bulls (n=30). Fourteen immunoreactive bands in capacitated spermatozoa of bulls (135, 100, 75, 70, 65, 55, 48, 45, 33, 31, 26, 24, 20 and 18 kDa) were detected in western blots following incubation with anti-HBP antibod...
متن کاملEffect of the Addition of Insulin Like Growth Factor-I (IGF-I) on Cryopreservation of Hariana Bull Semen
This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently su...
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ژورنال
عنوان ژورنال: Asian Journal of Advanced Research and Reports
سال: 2020
ISSN: 2582-3248
DOI: 10.9734/ajarr/2020/v9i330219